Device

Part:BBa_K3937007:Design

Designed by: Xuanshuo Zhang   Group: iGEM21_NPU-CHINA   (2021-10-01)


T7+Lac_Operator+BADE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 485
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 485
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 70
    Illegal XhoI site found at 923
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 485
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 485
    Illegal AgeI site found at 242
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is the part we used to test the information of BADE, we build it into pET28a to express the enzyme.


Source

It is from BADE and pET28a

References